PCR-based markers for resistance genes and tomato chromosomes
MERC/USAID Project: Development of tomatoes with combined resistance to TYLCV using both virus-derived resistance and molecular marker-assisted breeding
Activities: Cairo Meeting, Nov. 2006; Morocco meeting, Nov. 2007; Jordan meeting, Nov. 2008
This project will (i) evaluate several experimental breeding lines for resistance to TYLCV and TYLCSV in Middle Eastern countries, (ii) develop hybrids for local production with resistance to begomoviruses, (iii) develop methods for tagging the resistance genes, (iv) apply marker-assisted breeding methods for cultivar development, and (v) combine rDNA strategies with traditional sources of resistance.
Project duration: November 2001 to June 30, 2009.
Project coordinator: Dr. Hanokh Czosnek, Professor, Department of Field and Vegetable Crops, The Hebrew University of Jerusalem, Rehovot, Israel. Phone: 972-8-9489249; FAX: 972-8-9468265; E-mail: czosnek@agri.huji.ac.il.
Participating Countries: Egypt, Jordan, Israel, Lebanon, Morocco, Palestinian National Authority, Tunisia, University of Wisconsin-Madison, USA
CDR/USAID project: Develop tomato breeding lines with resistance to Ralstonia solanacearum and begomoviruses for Guatemala and Central America
Project No. C25-037
Whitefly-transmitted geminiviruses and bacterial wilt (BW) caused by Ralstonia solanacearum are perhaps the two most destructive and difficult tomato diseases to manage worldwide. In Guatemala, both diseases are currently responsible for substantial losses in tomato production. The most successful strategy for the control of these diseases is the use of resistant cultivars, along with appropriate cultural practices. A wide range of tomato germplasm with resistance to begomoviruses and bacterial wilt will be evaluated. Sources of resistance to these two diseases will be identified and combined in order to produce tomato cultivars with resistance to both pathogens. These cultivars will also be selected to possess good adaptation to local conditions and market quality. The population structure of R. solanacearum in the test area will be determined and resistant plants will be screened for resistance through inoculation with a mixture of all strains. As an immediate alternative for production in infested soils, elite susceptible cultivars will be grafted onto different sources of resistant rootstock. Individual loci conditioning resistance to the local begomoviruses and strains of R. solanacearum will be identified in the tomato genome and mapped using AFLP methods. Specific primers, to amplify molecular markers linked to these genes for resistance, will be developed to be used in marker-assisted selection in populations segregating for both types of resistance.
Project coordinators:
Dr. Hanokh Czosnek, Professor, Department of Field and Vegetable Crops, The Hebrew University of Jerusalem, Rehovot, Israel. Phone: 972-8-9489249; FAX: 972-8-9468265; E-mail: czosnek@agri.huji.ac.il.
Dr. L. Mejia, Professor, Faculty of Agronomy, San Carlos University, Guatemala City, Guatemala. Address: PO Box 590808, P-15, Miami, FL 33159 . Phone/Fax: 502-2-442-0588; mobile: 502-5-901-1468; E-mail: lmejia@gold.guate.net.gt
Project dates: March 2006 to March 2010
Participating Institutions : San Carlos University, Guatemala; Hebrew University of Jerusalem and Volcani Center, Israel; University of Florida and University of Wisconsin-Madison, USA; Asian Vegetable Research and Development Center, Tiawan.
CDR/USAID project: Molecular Marker-Assisted Breeding for Resistance to Whitefly-Transmitted Geminiviruses Infecting Tomato in Guatemala
This project will (i) evaluate experimental breeding lines for resistance to begomoviruses in Guatemala, (ii) develop molecular markers for resistance genes, (iii) apply marker-assisted breeding methods for cultivar development, (iv) characterize begomoviruses associated with tomatoes in Guatemala, and (v) incorporate marker-assisted breeding methods into the Biotechnology M. Sc. degree program.
Project coordinators:
Dr. Hanokh Czosnek, Professor, Department of Field and Vegetable Crops, The Hebrew University of Jerusalem, Rehovot, Israel. Phone: 972-8-9489249; FAX: 972-8-9468265; E-mail: czosnek@agri.huji.ac.il.
Dr.
L. Mejia, Professor, Faculty of Agronomy, San Carlos University, Guatemala City,
Guatemala. Address:
PO Box 590808, P-15, Miami, FL 33159 or 30 Calle, 9-23, Zona 11, 01011
Guatemala. Phone/Fax:
502-442-0588; E-mail: lmejia@gold.guate.net.gt
Project finished March 31, 2006
Participating Countries: Guatemala, Israel, USA
CDR/USAID Project and University of West Indies: Breeding tomatoes for resistance to TYLCV in Jamaica
This project involves the evaluation in Jamaica of germplasm selected for resistance to TYLCV in Israel and the development of hybrids with high levels of resistance to TYLCV and acceptable horticultural characteristics for Jamaica.
Project coordinators:
Dr. Hanokh Czosnek, Department of Field and Vegetable Crops, The Hebrew University of Jerusalem, Rehovot, Israel. Phone: 972-8-9489249; FAX: 972-8-9468265; E-mail: czosnek@agri.huji.ac.il.
Dr. Favi Vidavski, Vegetable Crops, and Genetics, Faculty of
Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel. E-mail: vidavaski@agri.huji.ac.il.
Dr. Wayne McLaughlin, University of West Indies, Mona, Kingston. E-mail: wmclaugh@uwimona.edu.jm.
Project finished
Participating Countries: Israel, Jamaica, USA
Created June 6, 2001; Upated August 2008. Contact: Douglas P. Maxwell (dpmax--plantpath.wisc.edu)