Sequence of one end of PCR insert of top A from the Elios sample:

    (Sequence has the EcoRI site and the vector sequence for the pGEM T-Easy plasmid from Promega, Inc. at the 5' end of the sequence.  The next sequence is either the PCR primer sequence for PAR1c715 or PAV1v1978.)

        (Feb. 23, 2000; Sequence prepared by Steve Hong)

GNAGNGGGGNTTTTGAACGCTTTGACGTCGCATGCTCCCGGCCGCCATGGCGGCCGCGGGA

ATTCGATTTTGATGTTCTCGTCCATCCATATCTTACCGAGGTTNTACACAGACTTAACACAGA

ACCGCTTACCAACACGGTGGGTGATACCATTACCACGTGTGACATCGGATATGCACATGACT

TTCCCCACGTGGGAGATATCGTGTCGTTGCTCGTAGGACTGGACCTTACATGGGCCTTCACAG

CCTCTTGGAACATCGGGCGTTCTCCAAGCCCGATATATCCTGGGCTTCCTATACATGGGCCTG

TTTACCCAGGCATGGGCCTTATCAAATTTAGGCCCAGTAGCAGTCCGGGGAGACTGGTTAGAG

GACCGACTCACCTTAGGGGTACCTGCCATTAAGCGCCAAGGGGCATCACGCTTAGGCATTATC

AATATTTGCGTGGTTGGACCACACACATAACGACAAATTTATAGACCATAAAACAACTTGGTGA

CCAAGTTAGTAATAATTAGACTACCCAAACGTGTACTGATTGGTTAACTTTATCCAGGAAGGCC

CAACGCAATTTCTGATAGTGGGCCAGGTCCAAAAAAAATCGCGGGCATCCGGTAATATTATTG

CGGATGGNCGAATTGCCCTTGGTGTCCCCAGAGGGGTTTAAGTATAAATGGGACACCAGGACA

CCACACATAGAGTTTAGAGAGAGCTNCCCTGGNGGTCTGGTCATATTTACAAAAATGGCACGG

AACCCTAATTTATTTCGTCTANCTGCCAAAATATTTTNTTACATA

Questions: 

    1)  Can you find the PCR primer sequence in this sequence?

    2)  Is this sequence in viral sense or complementary sense polarity?

    3)  This sequence matches what geminivirus gene or promoter region?

    4)  Is this an uncharacterized geminivirus or characterized geminivirus?  Why?

   5)  What is the vector for this geminivirus and how do you know this from the sequence data?

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