Table 5. Begomoviruses detected in samples from the tomato breeding program in Guatemala using PCR and hybridization protocols

1 M82, XA175 (Gh13 x Gc16), XA176 (Gc16 x M82), XA188 (Gh13 x M82); and commercial hybrids, Tango, Elios, Marina, and Silverado. M82 is susceptible to begomoviruses; Gc16 and Gh13 are begomovirus-resistant breeding lines (Mejía et al., 2004).
2Thirty-days-after transplanting; DSI, 0 = no symptoms, 1 = possible symptoms, 2 = slight symptoms, 3 = strong symptoms, 4 = severe symptoms
3Positive hybridization signal/PCR fragment
4CP-General probe/Degenerate primer set (PRepv1978/PCPc715

 

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